CCR2 is critical for the recruitment of proangiogenic monocytes/macrophages into skin wounds. (A) Scheme illustrating skin wound histology. (B-D) CD31 immunostaining (B) and morphometric quantification of CD31-stained (C) or H&E-stained (D) wound tissue at different time points after injury. (E-F) qRT-PCR analysis for VEGF in F4/80⁺CD11b⁺eGFP⁺ macrophages isolated from CCR2^fl/D mice normalized to expression in SSC^lowCD11b⁺CD115⁺ blood monocytes (E) and in wound tissue from mutants at different time points after injury normalized to unwounded skin (F). (G) Morphometric quantification of αSMA-stained wound tissue of the indicated mouse strains. Three independent experiments were performed and each dot represents 1 wound. Hatched line indicates granulation tissue; dotted line, hyperproliferative epithelial tongue; arrows, tip of epithelial tongue; d, dermis; g, granulation tissue; he, hyperproliferative epithelium; sc, subcutaneous fat tissue; pc, panniculus carnosus; and sm, skeletal muscle. Bars indicate 100 μm. *P < .05; **P < .01; ***P < .001.