Clonal heterogeneity determined by single cell analysis. (A) Mutant allele frequencies of 47 exonic synonymous and NS variants identified through exome sequencing. The frequencies of 4 variants are shown. (B) Proportion of cells identified with a mutated genotype for 4 genes as determined by exome sequencing allelic frequencies and single cell genotyping. (C) Hierarchical clustering of the genotypes generated from the analysis of 270 single cells. Grey indicates mutated; and black, reference. Individual populations are identified (1-4). (D) Determined cell lineages showing the proportion of tumor population and genotypes for the 4 genes in the identified populations (mutated genotypes underlined).