Newly generated iTreg cells protect from autoimmunity. (A) DC-tg Rag2^−/− (DC-tg) or Rag2^−/− control (ntg) mice were adoptively transferred with polyclonal CD4⁺ DEREG T cells. Three weeks after transfer, recipient mice treated or not with diphtheria toxin were infected with HSV-1 and incidence and severity of the disease scored on days 7 and 10 after treatment. Note that deletion of Foxp3⁺ cells in the recipient DC-tg mice makes them strongly susceptible to HSK. Data are mean ± SEM of 6 mice per group. (B) Left panels: percentage of CD25⁺Foxp3⁺ on CD4⁺CD45⁺ cells present in the corneas of DC-tg Rag2^−/− (DC-tg) or Rag2^−/− (ntg) recipient mice at day 10 after HSV-1 ocular infection. Right panel: quantification of the cytofluorimetric analysis. Data are mean ± SEM calculated on 3 different mice. ****P < .001.