Expansion of Mll^PTD/WTmice HSPCs in CFU-spleen and replating assays. (A) Representative image of CFU-spleen assay. (B) Number of colonies was counted 8 or 12 days after transplantation (12 mice per group; 1 × 10⁵ cells per mouse). *P < .05. **P < .01. (C) Diameter of each colony was measured under inverted microscope. (D) Frequency of CFU-Cs in the BM of WT and Mll^PTD/WT during serial replating on methylcellulose in vitro. (E) Proportion of CFU-Cs in serial replating. (F) Representative image of colonies from WT and Mll^PTD/WT BM cells in the second round of replating.