Phenotypic ST-HSCs, MPP, and GMP from Mll^PTD/WT mice repopulate LT-HSCs. Representative FACS contour diagram shows the repopulation of LSK, LT-HSC/ST-HSC/MPP, and CMP/GMP/MEP in recipients transplanted with Mll^PTD/WT different fractions, Mll^PTD/WT LT-HSCs (A), ST-HSCs (B), MPP (C), and GMP (D). (E) Mean ± SD percentage of donor-derived Mll^PTD/WT cells (CD45.2⁺) in recipient BM. (F) Frequency of donor-derived lineage-repopulation myeloid or B or T cells compared with competitor (CD45.1) derived lineage-repopulation present in recipient BM. Data are at 12 weeks after transplantation (n = 4). (G) Secondary transplantation was performed 16 weeks after primary transplantation with sorted fractions from Mll^PTD/WT MPP transplanted recipients (n = 4). Data are mean ± SD percentage of donor-derived Mll^PTD/WT cells (CD45.2⁺) in recipient PB. (H) Frequency of donor-derived lineage-repopulation myeloid or B or T cells compared with competitor (CD45.1) derived lineage-repopulation present in secondary recipient PB 12 weeks after transplantation.