L-selectin ligand expression in St3gal4-deficient, St3gal6-deficient, and double-deficient mice. (A) Frozen sections of ALNs, ILNs, MLNs, and PPs were stained with L-selectin–IgM (green) and αtubulin Ab (red). Serial sections were stained with H&E. Scale bars indicate 50 μm. (B) Scattergrams indicate the frequencies of L-selectin–IgM- and αtubulin-positive cells quantified using TissueQuest Version 3.0.1120.0136 microscope software systems. Data are representative of 10 independent experiments.