Validation of a 3-gene model classifier by RT-qPCR in cryopreserved samples. (A) Box plot showing RT-qPCR quantification of TNFRSF8, BATF3, TMOD1, TMEM158, GZMB, and PRF1 expression in PTCL-NOS and ALK⁻ ALCL. Statistical significance calculated using the Wilcoxon rank-sum test are shown. Expression levels are given as ΔCt. (B) Classification accuracy measures. positive predictive value, 94.7%; negative predictive value, 93.3%.