Validation of the 3-gene model classifier by RT-qPCR in FFPE tissues. (A) Box-plot and (B) point-wise distribution showing RT-qPCR quantification of TNFRSF8, BATF3, and TMOD1 expressions in FFPE samples of PTCL-NOS (n = 32) and ALK⁻ ALCL (n = 34), together with the predicted output using LDA and leave one-out procedure. P indicates PTCL; and A, ALCL. (C) Classification accuracy measures. positive predictive value, 96.9%; negative predictive value, 97.1%; (D) Box-plot and strip-chart distributions of the metagene values. Gray band indicates the unclassifiable samples area.