Differentiation and characterization of iPSCs-derived macrophages. (A) KDR⁺ CD34⁺ hematopoietic progenitors purified 10 days after differentiation. (B) The percentage of KDR⁺ CD34⁺ cells in Tra-1-85⁺ human cells. n = 3. (C) CD68 immunostaining of macrophages. Scale bars represent 100 μm. (D) The histograms show antibody staining (in black) relative to the isotype-matched controls (in white) for a blood cell marker (CD45), and a macrophage marker (CD14), in cells before (left 2 panels) or after (right 2 panels) magnetic-activated cell sorting purification. (E) CD14⁺ cell counts obtained from iPSCs plated on an OP9 feeder layer on one 100-mm dish. n = 3. (F) Representative morphology of iPS-MPs evaluated by May-Giemsa staining or transmission electron microscopy. Scale bars represent 100 μm and 2 μm, respectively. (G) The phagocytosis by iPS-MPs after LM infection. The cells were treated with anti-LM antibody, phalloidin, and 4′,6-diamidino-2-phenylindole. Scale bar represents 20 μm. (H) The percentage of iPS-MPs phagocytosing LM was calculated as the average of 9 fields of vision. Data are mean ± SEM.