Remodeling mosaicism by coculturing mutant and wild-type iPS-MPs. (A) IL-1β secretion from cocultured iPS-MPs. We used 2 × 10⁴ mutant iPS-MPs (M1) and 8 × 10⁴ wild-type iPS-MPs (W1) as indicated. n = 6. (B) IL-1β secretion from various numbers of mutant (left panel) or wild-type (right panel) iPS-MPs. The iPS-MPs were seeded at the indicated numbers. n = 3. (C) IL-1β secretion from iPS-MPs that were cocultured at various ratios. The wild-type or mutant iPS-MPs were seeded at the numbers indicated in the first and second rows, respectively. The percentage of mutants is indicated in the third row; n = 3. (D) Increase of IL-1β levels during stimulation by the supernatant. The supernatant was harvested from the wells of the indicated iPS-MPs (Sup) and transferred to the wells of other iPS-MPs (Cell); n = 3. (E) IL-1β secretion from cocultured iPS-MPs in the presence of the ATP receptor antagonist, oATP (300μM) or PPADS (300μM). We used 2 × 10⁴ mutant iPS-MPs (M1) and 8 × 10⁴ wild-type iPS-MPs (W1) as indicated. n = 6. Data are mean ± SEM. ***P < .001 (Student t test). **P < .01 (Student t test). *P < .05 (Student t test).