Effect of LOX inhibition on marrow fibrosis in vivo. (A) Representative hematoxylin and eosin (left column) and Gomori silver (right column) staining of longitudinal sections of femurs from wild-type and GATA-1^low (male littermates), control, or BAPN-treated mice (10.5 weeks old at the time of collection). Original magnifications: left column, ×400; and right column, ×600. Arrows indicate the large presence of MKs (hematoxylin and eosin stain) and the accumulation of reticulin fibers in the GATA-1^low mice. Images obtained using Nikon Eclipse 50i microscope equipped with a 40× Plan (numerical aperture 0.65) and a 60× Plan Fluor (numerical aperture 0.85) objectives. Digital images acquired using a SPOT 2 megapixel camera and SPOT 5.0 acquisition software. (B) Quantification of fibrosis in BAPN or vehicle-treated GATA-1^low mice. Fibers were measured in arbitrary units from stained sections. Data are represented as absolute values (top panel) or as mean percent change compared with values recorded from vehicle-treated GATA-1^low mice (bottom panel). The mean values were obtained from 5 mice per group. **P < .05. Reproduced from Eliades et al⁷⁵ with permission.