ICAM-1 expression, neutrophil (PMN) infiltration into the lung, and activation of Src and eNOS in carrageenan-treated mouse lungs. (A) Mice were injected in the pleural space with 1% carrageenan or saline (sham); and after 4 hours, lung tissue was collected and analyzed. ICAM-1 expression was determined by Western blot and densitometry (mean ± SD; n = 3, lung tissues from 3 mice in each group). *P < .05 versus sham. (B) Activation of Src and eNOS was determined by Western blot analysis. Tyrosine phosphorylation of Src at Y418 and serine phosphorylation of eNOS at S1177 were detected (triplicates are shown) by Western blotting mouse lung homogenates. *P < .05 versus sham.