Collagen- and FcγRIIA-dependent platelet aggregation according to CD148 Q276P/R326Q polymorphisms. (A-B) Washed platelets isolated from healthy heterozygous FcγRIIA 131H/R donors (n = 20) were incubated with murine IgG1 monoclonal antibodies ALB6 (2.5 μg/mL) and PL2-49 (7.5 μg/mL). Lag time (A) and maximum aggregation (B) were analyzed according to CD148 polymorphisms (▩ noncarriers = 13 and ▨ carriers of the 276P/326Q alleles = 7). (C-D) Washed platelets isolated from healthy donors (n = 33) were incubated with 0.5, 1, or 10 μg/mL collagen or with 0.05 IU/mL thrombin. Lag time (C) and maximum aggregation (D) were analyzed in relation to CD148 polymorphisms (▩ noncarriers = 21 and ▨ carriers of the 276P/326Q alleles = 12). The horizontal lines in the middle of box-and-whiskers plots show the median values. The tops and bottoms of boxes show the 25th and 75th percentiles. The whiskers define the 10th and 90th percentiles. Mann-Whitney U test was performed to compare aggregation parameters in relation to polymorphisms of CD148, and significant P values are displayed.
