iMC expand upon autochthonous tumor development. Single-cell preparations of spleens were stained with antibodies against CD11b and Gr-1. Spleens were derived from young LoxP-Tag mice (Tg, 2-4 months), LoxP-Tag mice bearing sporadic tumors (Tg; kidney tumor n = 7, HCC n = 5, colon tumor n = 4, others n = 3), HCC-bearing LoxP-Tag × Alb-Cre mice (Tg × Alb-Cre), and Ad.Cre-infected LoxP-Tag mice bearing virus-induced HCC (Tg/Ad.Cre). (A) One representative example per experimental group is shown; numbers indicate the percentage CD11b⁺Gr-1^high and CD11b⁺Gr-1^int iMC of total spleen cells. All data are summarized in (B-D). The percentage of total CD11b⁺ cells (B), CD11b⁺Gr-1^int iMC (C), and CD11b⁺Gr-1^high iMC (D) in spleens of young Tg mice, tumor-bearing Tg mice, Tg × Alb-Cre mice, and Tg/Ad.Cre mice is indicated. Horizontal bars show means; error bars represent SEM. Overall significance for each graph was tested by Kruskal–Wallis 1-way analysis of variance.