iMC from mice bearing Tag-driven autochthonous tumors do not transfer CTL suppression in vivo. (A, B) The suppressive potential of CD11b⁺ cells (A) and CD11b⁺Gr-1^int iMC (B) from LoxP-Tag mice bearing sporadic tumors (Tg), HCC-bearing LoxP-Tag x Alb-Cre mice (Tg × Alb-Cre), and Ad.Cre-infected LoxP-Tag mice bearing virus-induced HCC (Tg/Ad.Cre) was analyzed upon cell transfer into B6 mice, immunized with Tag pIV in incomplete Freund's adjuvant. In vivo cytotoxicity against Tag peptide IV was analyzed by flow cytometry. The percentage of specific killing in vivo is indicated. Experimental groups were no iMC (ø, n = 7), iMC from Tg mice (n = 3), iMC from Tg × Alb-Cre mice (n = 5), and iMC from Tg/Ad.Cre mice (n = 4). Bars represent means; error bars indicate SEM.