Slow restoration of CD4⁺ T cells correlates with slow restoration of FRC and FDC networks. (A) Immunofluorescent staining of CD4 and desmin in LNs from RMs before and at different time points after stopping CD4-depleting antibody treatment, showing that the slow restoration of CD4 T cells after stopping antibody treatment correlates with slow restoration of FRC and FDC networks. (B) Quantitative image analysis of the number of CD4 T cells in LNs and the percent area of stromal cells, showing the slow restoration of both CD4 T cells and stromal cells. Bars represent the mean ± SEM; and the dotted lines, the timing of anti-CD4 antibody administrations. All the comparisons are made between labeled time point and predepletion. Star color matches line color. *P < .05. **P < .01. ***P < .001.