Deletion of CCX-CKR results in enhanced spontaneous autoimmunity characterized by lymphocytic infiltrate in 8- to 10-month-old mice. (A) Quantitation of the average number of lesions identified in 3 sections per gland cut ∼ 100 μm apart, with the second section being the approximate middle of the tissue. WT, n = 16; CCX-CKR^−/−, n = 14; and CCR7^−/−, n = 7. Bars represent means. (B) Serial sections of submandibular glands were stained with hematoxylin and eosin (H&E) to identify lesions (indicated with arrows). Boxed region indicates areas shown below stained with anti-CD4, anti-CD8, anti-CD11c, and anti-CD45R to identify infiltrating cell populations. (C) Same as panel A, but in the liver. (D) Serial sections of the liver stained with H&E to identify lesions. Boxed region represents areas shown below stained with anti-CD4. H&E scale bar represents 400 μm. IF scale bar represents 250 μm. (A,C) One-way ANOVA with Bonferroni posttest. *P < .05. **P < .01. ***P < .001.