Decreased levels of chemokines and disruption to CCL25 distribution in CCX-CKR^−/− thymi. (A) Levels of CCL19, CCL21, and CCL25 were measured by ELISA in thymus homogenates. Data are pooled from 3 independent experiments; n = 15 or 16. Two-tailed unpaired t test with Welch correction: *P = .0184, **P = .0014. Bars represent means. (B) Analysis of chemokine distribution in thymus sections stained with anti-CD44 cells (green) to indicate the medulla and anti-CCL21 (red). Visualization of CCL21 distribution (right) by plotting CCL21 pixel intensity versus distance from the CMJ. Representative IF images from 2 independent experiments: WT, n = 6; and CCX-CKR^−/−, n = 6. (C) Same as panel B, but stained with anti-CCL25 (red). Representative IF images from 2 independent experiments: WT, n = 7; and CCX-CKR^−/−, n = 9. CCL21 and CCL25 distribution data pooled from analysis of the corresponding sections with 2 fields of view per thymus. Scale bar represents 250 μm.