American Society of Hematology

Figure 5

$Figure 5. TCR- versus cytokine-dependent signaling events. (A-D) Representative flow cytometric analysis of phosphoproteins in fractionated DP thymocytes cultured for with peptide-pulsed OP9 cells for 24 hours in medium supplemented with rIL-4, rIL-7, rIL-13, or rIL-21 (red histograms) or no cytokines (black histograms). (E) DP thymocytes were cultured in medium containing rIL-4, rIL-7, rIL-13, rIL-21, or no cytokines with (red) or without (black) EIINFEKL-pulsed OP9 cells. For panels A through E, 3-6 mice per group were tested in separate experiments. (F) qPCR analysis of miR-181a expression in freshly harvested thymocyte subsets from OT-I mice. Data depict transcript abundance relative to DN thymocytes. (G) qPCR analysis of miR-181a in DP3 thymocytes cultured for 24 hours in cytokine-supplemented medium in the presence or absence of peptide-pulsed OP9 cells. In panels F and G, 3-7 mice per group were tested. *P < .05; **P < .01.$

TCR- versus cytokine-dependent signaling events. (A-D) Representative flow cytometric analysis of phosphoproteins in fractionated DP thymocytes cultured for with peptide-pulsed OP9 cells for 24 hours in medium supplemented with rIL-4, rIL-7, rIL-13, or rIL-21 (red histograms) or no cytokines (black histograms). (E) DP thymocytes were cultured in medium containing rIL-4, rIL-7, rIL-13, rIL-21, or no cytokines with (red) or without (black) EIINFEKL-pulsed OP9 cells. For panels A through E, 3-6 mice per group were tested in separate experiments. (F) qPCR analysis of miR-181a expression in freshly harvested thymocyte subsets from OT-I mice. Data depict transcript abundance relative to DN thymocytes. (G) qPCR analysis of miR-181a in DP3 thymocytes cultured for 24 hours in cytokine-supplemented medium in the presence or absence of peptide-pulsed OP9 cells. In panels F and G, 3-7 mice per group were tested. *P < .05; **P < .01.

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