Expression of SOX4 target genes in ATL. (A) Gene expression analysis. Semiquantitative RT-PCR was performed to analyze expression of GCKR, NAP1, and HDAC8 in ATL cell lines and Raji (top panel), and in normal CD4⁺ T cells and PBMCs from ATL patients (>90% leukemia cells) (bottom panel). PHA-PBMC, normal PBMCs treated with PHA for 3 days. GAPDH was used as a loading control. The representative results from 3 separate experiments are shown. (B) Effect of SOX4 siRNA on target gene expression. Two ATL cell lines (HUT102 and ST1) were transfected with control siRNA or SOX4 siRNA. After 48 hours, total RNA samples were prepared. Quantitative real-time PCR was performed for GCKR, NAP1, HDAC8, and GAPDH. The expression levels were normalized by GAPDH. Data are shown as mean ± SEM of 3 separate experiments. *P < .05; **P < .01.