Purified band cells gain surface expression of CD11c and MHC II in GM-CSF–supplemented culture. (A) Gr-1^high/CD48⁻ band cells purified from BM of C57BL/6 mice (CD45.2) were examined for surface expression of the indicated markers. Blue lines indicate staining profiles with isotype-matched control IgG. (B-D) Band cells purified from C57BL/6 mice were cultured for 4 d (B) or 6 d (C) with BM feeder cells from B6 SJL mice (CD45.1) in the presence of GM-CSF, G-CSF, M-CSF, or Flt3L. The data show the expression profiles of CD11c (top panels) and Ly6G (bottom panels) within the CD45.2⁺ gated population and the recovery rates compared with the originally plated band cell number. (D) The data show the expression profiles of CD11c, MHC II, and Ly6G within the CD45.2⁺/CD45.1⁻ gated population harvested on d 6 from GM-CSF–supplemented co-cultures. Data are representative of at least 3 independent experiments.