Identification of unique properties that distinguish neutrophil-DC hybrids from traditional DCs. (A) Ly6G⁺/CD11c⁺/MHC II⁺ neutrophil-DC hybrids and Ly6G⁻/CD11c⁺/MHC II⁺ traditional DCs purified from the same GM-CSF–supplemented BM cultures (d 6) were compared for global gene expression profiles. The heat map was created from 3 independent pairs to show the genes that are predominantly expressed (>2-fold and P < .05). The whole-gene datasets have been deposited in Gene Expression Omnibus with accession number GSE28408. (B-D) Ly6G⁺/CD11c⁻/MHC II⁻ neutrophils, Ly6G⁺/CD11c⁺/MHC II⁺ hybrids, and Ly6G⁻/CD11c⁺/MHC II⁺ traditional DCs were examined for MMP9 mRNA expression by quantitative polymerase chain reaction and MMP9 protein elaboration by enzyme-linked immunosorbent assay (means ± SD from triplicate samples) (B) and for surface expression of CD62L (C) and CXCR2 (D). Data are representative of 3 independent experiments.