Visualization of the carotid artery endothelial surface by SEM. (A) The image shows a longitudinal section from an untreated mouse, approximately 1.5 mm long. (B-C) The images are of the carotid artery 1 minute following FeCl₃ application, visualized by SEM. Thrombotic material can be observed covering much of the endothelial surface. Higher magnification of the area from (B, box) is shown in (C). (D) The fluorescent intravital microscopy image shows FeCl₃-induced thrombosis in the mouse carotid artery. Platelets were observed to bind in clusters rather than discretely. The time frame is approximately 30 seconds following the application FeCl₃. *, platelet clusters (not all are marked).