PDK1 is involved in integrin αIIbβ3-mediated outside-in signaling. (A) Binding of Rhodamine-Fg to PDK1^f/f and PDK1^−/− platelets stimulated by a low dose of thrombin. (B) Phosphorylation of Akt Thr308 (T308), Akt Ser473 (S473), and Gsk3β Ser9 (S9) in PDK1^f/f and PDK1^−/− platelets stimulated by 0.05 U/mL of thrombin in the absence or presence of αIIbβ3 blocking mAb 1B5. (C) Spreading of PDK1^f/f and PDK1^−/− platelets on immobilized Fg in the presence of dimethylsulfoxide, 10 μM SB, 100 nM Wort, or 10 μM SB plus 100 nM Wort. (D) Quantification of the areas (pixel number) of 4 random fields (mean ± standard error of the mean). Statistical analyses were performed using the Student t test. (E) Phosphorylation of Akt T308, Akt S473, and Gsk3β S9 in PDK1^f/f and PDK1^−/− platelets spread on Fg in the presence of dimethylsulfoxide, 10 μM SB, 100 nM Wort, or 10 μM SB plus 100 nM Wort.