Locations of 61 TP53 mutations identified in 52 of 457 CLL patients. (A) Organization of the human TP53 gene. Noncoding exons are shown in gray, coding exons in black. TP53 splice mutations are indicated by arrows. (B) TP53 protein with known functional domains. NES, nuclear exclusion domain; NLS, nuclear localization domain; TAD, transactivation domain. Dominant-negative TP53 mutations (according to the IARC database) are shown in bold with an asterisk. ●, missense, *dominant negative; ○, nonsense; ▪, in-frame deletion; □, frameshift deletion; ▼, in-frame insertion; ▿, frameshift insertion; ↓, splice-site mutation.