HPCs express TF and contribute the majority of TF-dependent PCA in liver. (A) Representative agarose gel visualization of a TF PCR amplicon from primary HPCs isolated from control mice (TF^flox/flox mice) or HPC^ΔTF mice. HPRT is shown for loading. M: DNA ladder. (B) TF-dependent PCA from lysates of control or HPC^ΔTF HPCs. Data are expressed as mean ± SEM relative to control HPCs (n = 3 independent experiments). *Significantly different from control HPCs (P < .05). (C) PCA of liver homogenates from control mice, HPC^ΔTF mice, and low TF mice. Data are expressed as mean +SEM relative to control mice (n = 3-5 mice/group). *Significantly different from control liver homogenates (P < .05).