IFN-γ inhibits HSC recovery after LCMV infection. (A) WT and IFN-γ^−/− mice were infected with LCMV and the number of HSCs (Lin⁻c-Kit⁺CD48⁻CD150⁺) was measured by flow cytometry at indicated days (n = 3-5). Data represent 3 independent experiments. Mixed BM-chimeric mice were generated with WT (CD45.1) and IFN-γR1^−/− (CD45.2) BM (1:1 ratio), infected with LCMV, and donor HSC chimerism (B), donor HSC numbers (C), and proliferation of donor HSCs (D) were measured at indicated days (n = 3-5). Data are representative of 2 independent experiments. Mean values ± SEM are shown. *P < .05; **P < .01; ***P < .001.