Kinetics of pDC development in FL BM cultures. BM depleted of erythrocytes and BST-2⁺ cells (A), CLPs (B), or CMPs (C) were cultured with FL. The number of CD11c⁺CD45RA⁺ pDCs or CD11c⁺ CD45RA⁻ cDCs was assayed each day by flow cytometry. (D) The CD11c⁺CD45RA⁺ pDC progeny of CLPs and CMPs sorted as in panels B and C were analyzed by flow cytometry for the expression of the pDC markers BST-2 (CD317), CD45R (B220), and Siglec H. Results are representative of 3 separate experiments.