RAG1 expression in pDCs from lymphoid and myeloid progenitors. CLPs (A) or CMPs (B) were isolated from the BM of RAG1-GFP reporter mice and cultured with FL and congenic BM feeder cells. After 5 days, pDCs were identified as CD11c⁺CD45RA⁺ and their GFP expression assessed by flow cytometry. Results are representative of 2 (A) or 6 (B) independent experiments.