RAG1 expression in CMP and CMP-derived pDCs. CMPs were isolated from the BM of RAG1-GFP reporter mice and analyzed by for expression of flt3 and RAG1 (A), Flt3⁺ RAG1⁺ (B), or flt3⁺RAG1⁺ and flt3⁺RAG1⁻ CMP were sorted from the BM of RAG1-GFP reporter mice and cultured with GM-CSF (B; 10 ng/mL) or FL (C). (B) Progeny were analyzed after 7 days and macrophages identified as CD11b^highF4/80⁺. (C) After 5 days, pDCs were identified as CD11c⁺ BST-2⁺ and cDCs identified as CD11c⁺BST-2⁻. The GFP expression of RAG1⁺ CMP-derived cDCs (light gray shaded histogram) and pDCs (dark gray shaded histogram) and RAG1⁻ CMP-derived cDCs (dashed line) and pDCs (solid line) was analyzed by flow cytometry. Plots are representative of 5 (A), 2 (B), or 4 (C) independent experiments.