Torin1-conditioned DCs are predominantly CD86^loB7-H1^hi. (A) Analysis of the coexpression of CD86 and B7-H1 on CD11c-gated cells with percentage of CD86^loB7-H1^hi DCs indicated in the gate. (B) Quantification of the frequency of CD86^loB7-H1^hi DCs across multiple experiments. (C) Ratio of the normalized B7-H1 MFI divided by the normalized CD86 MFI for the indicated culture conditions. n ≥ 5 experiments for all data presented. *, # P < .05 when compared with DMSO and RAPA, respectively.