TLR4 and TRIF are required for the induction of anti-Aβ IgG response by Aβ-peptide liposomes. (A) Anti-Aβ IgG and (B) IgM antibodies in the plasma of WT mice receiving Aβ-peptide liposomes (26 μg of PalmAβ1-15) with or without 30 μg of MPLA (as indicated by arrows). IgG results are expressed as mean ± SD, while IgM results are expressed as mean OD values at a dilution of 1/100 + SD (n = 10 per group). The data are representative of 2 independent experiments. (C) Anti-Aβ IgG and (D) IgM antibodies in the plasma of WT, CD14^−/−, and TLR4^−/− mice immunized s.c. with Aβ-peptide liposomes (as indicated by arrows). IgG results are expressed as mean ± SD, while IgM results from day 7 are expressed as mean OD at a dilution of 1/100 + SD (n = 6 per group). (E) Analysis of anti-Aβ IgG subclasses in the sera of WT mice, CD14^−/− mice, and TLR4^−/− mice receiving Aβ-peptide liposomes. Results are expressed as mean OD values at a dilution of 1/400 + SD (n = 6 per group). (F) Anti-Aβ IgG antibodies in the sera of WT mice, MyD88^−/− mice, and TRIF^−/− receiving Aβ-peptide liposomes (as indicated by arrows). IgG results are expressed as mean ± SD (n = 6 per group).