Identification of neutrophil-DC hybrids in thioglycollate-induced peritonitis lesions. (A-B) After intraperitoneal injection of thioglycollate into C57BL/6 mice, PECs harvested on day 2 (A) or at the indicated time points (B) were analyzed for the expression of Ly6G, CD11c, and MHC II. Data shown are Ly6G and CD11c expression profiles (A) and the numbers of total cells in the PECs, Ly6G⁺/CD11c⁻/MHC II⁻ neutrophils, Ly6G⁺/CD11c^high/MHC II⁺ neutrophil-DC hybrids, and Ly6G⁻/CD11c^high/MHC II⁺ traditional DCs (B). (C) Ly6G⁺/CD11c⁻/MHC II⁻ neutrophils, Ly6G⁺/CD11c^high/MHC II⁺ neutrophil-DC hybrids, and Ly6G⁻/CD11c^high/MHC II⁺ traditional DCs purified from the PECs (day 2) were examined for morphology after HEMA-3 staining (bars represent 20 μm). (D) PECs harvested 2 days after thioglycollate treatment were examined for the expression profiles for the indicated leukocyte markers within the Ly6G⁺/CD11c⁻ neutrophil population, the Ly6G⁺/CD11c^high hybrid population, and Ly6G⁻/CD11c^high traditional DC population. Data are representative of at least 5 independent experiments.