Bacterial infection triggers the emergence of neutrophil-DC hybrids. (A) PECs harvested from C57BL/6 mice 24 hours after intraperitoneal injection of PBS alone or E. coli were examined for surface expression of Ly6G, CD11c, and MHC II. Data shown are staining profiles with anti-Ly6G mAb (red) or control IgG (blue) within the CD11c^high/MHC II⁺ gated populations. (B) The same PEC samples harvested after E. coli injection were examined for expression of the indicated leukocyte markers within the Ly6G⁺/CD11c^high gated population. Data are representative of 5 independent experiments.