Emergence of neutrophil-DC hybrids in various organs under inflammatory conditions. Single cell suspensions were prepared from ear skin samples harvested from KC-Tie2 TG mice or age-matched WT mice (3 mice per panel) (A), from lung samples harvested from BALB/c mice (3 mice per panel) 48 hours after intratracheal instillation of GC frass or PBS alone (B), or from skin-draining LN samples harvested from C57BL/6 mice (5 mice per panel) 24 hours after subcutaneous inoculation of live E. coli or PBS alone (C). Data shown are the numbers (means ± SD) of Ly6G⁺/CD11c⁻/MHC II⁻ neutrophils, Ly6G⁺/CD11c^high/MHC II⁺ neutrophil-DC hybrids, and Ly6G⁻/CD11c^high/MHC II⁺ traditional DCs. *P < .05; **P < .01 between the indicated samples. (D) The same LN samples harvested after E. coli injection were examined for expression of the indicated leukocyte markers within the Ly6G⁺/CD11c^high gated population. Data are representative of 5 independent experiments.