Acute effect of N8-GP in a tail bleeding model in FVIII-deficient mice. The mice received N8-GP or FVIII (Advate) intravenously at the indicated doses, and tail bleeding was measured after clipping 4 mm of the tail tip 5 minutes after dosing. Dose-response curves of total bleeding time (A) and blood loss (B) are shown for N8-GP (closed circles, black line) and FVIII (open diamonds, dotted lines). A blood loss of 1000 nmol hemoglobin approximates 0.125 mL whole blood using a hemoglobin concentration of 8 mmol/L. Control animals receiving the vehicle only are shown with open circles. Data are mean ± standard error of the mean (SEM) of n = 12 mice per group.