Prolonged effect of N8-GP in a joint bleeding model in FVIII-deficient mice. N8-GP (A, open bars; B, open circles) or FVIII (Advate) (A, solid bars; B, solid circles) were administered intravenously to the mice at 280 U/kg, and needle-induced joint bleeding in the knee induced at the time points indicated after dosing. The gray bar (A) or gray circle (B) correspond to FVIII-deficient mice, and the hatched bar (A) and solid square (B) to normal mice (C57BL/6). The bleeding was evaluated 24 hours after injury, and VBS (A) and change in joint diameter (B) determined. A VBS of 3 indicates tense and distorted knees, whereas 0 designates a virtually nonaffected knee. Data are mean and SEM of 10 mice per group at time points 5 minutes, 24 hours, 72 hours, and 88 hours, and 15 mice per group at 36 to 60 hours after injury. *P < .05; **P < .01; and *** P < .001 indicate significant difference between treated and nontreated FVIII-deficient mice. Above the bar in (A), ***P < .001 indicates significant difference between FVIII-deficient and C57BL/6 control mice.