Siglec-E associates with SHP-1 in the absence of tyrosine phosphorylation. WT and siglec-E^−/− bone marrow cells were plated on fibrinogen or stimulated in suspension with either pervanadate (PV) or with LPS (10 µg/mL) for the indicated time periods at 37°C. Lysates were immunoprecipitated with anti–siglec-E antibody followed by immunoblotting with anti–siglec-E, anti–SHP-1, anti–SHP-2, and anti-pY antibodies. As a positive control for siglec-E and SHP-2, lysates from WT and siglec-E–transfected CHO cells were analyzed in parallel. Data are representative of 3 independent experiments.