α-Camera imaging. (A) Cryosections (10 µm) of spleen imaged 3 hours after intravenous injection of ²¹¹At-B10-30F11. (B) The third section from the left in panel A was used for quantitative analysis of the activity distribution of ²¹¹At within the spleen. Each pixel intensity value (activity) was normalized to the mean pixel intensity of the whole spleen. The normalized data were divided in 10 bins (0 to max) and plotted as a color-coded histogram (1.0 = mean activity of the whole section). (C) Cryosections (10 µm) of femur imaged 3 hours after intravenous injection of ²¹¹At-B10-30F11. The large ROIs (yellow) outline the areas used for quantification of % ID/g in bone marrow by α-camera imaging. The small ROIs (magenta) in the middle femur image were used to compare the activity uptake (% ID/g) along the bone marrow cavity. (D) Quantitative histogram of ²¹¹At distribution of the middle femur section in panel C.