Characteristics of CTL product for 50 patients who received CTL infusion. (A) Cell-surface phenotype of CTL lines infused (n = 50). CD3⁺, CD19⁺, CD14⁺, CD3⁻/CD56⁺ and CD3⁺/CD56⁺ are shown as a percentage of total cells; CD4⁺, CD8⁺, CD45RO⁺, CD45RA⁺, CD62L⁺ and tetramer are shown as a percentage of CD3⁺. Tetramer represents the total percentage of tetramer-positive cells. For participants with a tetramer for more than one HLA allele, the sum of all available tetramers is shown. Bars and whiskers represent means and standard deviations. (B) Cytotoxic capacity and alloreactivity of CMV-specific CTL using the ⁵¹Cr assay. CMV-specific cytotoxic activity was measured by specific lysis of pretransplant recipient-derived PHA blasts pulsed with an overlapping peptide mix spanning the pp65 protein; alloreactivity was measured by specific lysis of unpulsed recipient PHA blasts. Values shown are specific lysis at an effector-to-target ratio of 20:1. Bars and whiskers represent means and standard deviations.