Platelet-decorated VWF strings rapidly appear on the surface of activated endothelial cells. (A) In vitro, platelet-decorated VWF strings are visualized by fluorescence microscopy on the surface of blood outgrowth endothelial cells perfused with washed DIOC6-labeled platelets at a shear rate of 250 seconds−1.43 Scale bar represents 50 μm. (B-C) Platelet-decorated VWF strings (white arrows) appear on the surface of mesenteric veins of Adamts13−/− mice after activation with 10% FeCl3 solution (B) or calcium ionophore A23187 (C). Endogenous platelets were labeled by intravenous administration of rhodamine 6G.42 Microscopy was performed using a Nikon Eclipse TE200 inverted fluorescence microscope equipped with a 20× PLAN objective (numeric aperture 0.4) coupled to an ORCA-R2 Hamamatsu CCD camera. Images were recorded using Hokawo software (Hamamatsu Photonics).
