CtBP1 interacts with FANCC in cells. (A) Co-immunoprecipitation of CtBP1 with FANCC. HEK293T cells were transfected with GFP-tagged FANCC constructs expressing full-length (FANCC^8-558) or truncated FANCC (FANCC ^55-558, FANCC^1-306, and FANCC^307-558) constructs. Whole cell extracts (WCEs) were subjected to immunoprecipitation (IP) with antibodies against GFP or CtBP1. Negative IP controls were performed using mouse immunoglobulin G (IgG) (M). IPs were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted (IB) with the indicated antibodies. Data shown are representative of at least 3 independent experiments. (B) Co-IP of endogenous FANCC and CtBP1 proteins from HEK293T cell extracts using anti-CtBP1 or anti-FANCC antibodies. Negative IP control was performed using rabbit IgG (R). Shown is 1 of 5 representative experiments. (C) CtBP1 and FANCC localization in HeLa cells. Cells were double-stained with anti-CtBP1 (green) and anti-FANCC (red) antibodies and were visualized via confocal fluorescence microscopy using a Nikon E800 microscope equipped with a C1 confocal system at 100× magnification. White boxes in the left panel indicate selected cells that have been magnified in the right panel. Data shown are representative of 3 experiments in which at least 25 cells were analyzed.