Prenatal manifestation of several FA-associated defects. (A) Fancc^+/− crosses yielded an approximately Mendelian frequency of Fancc^−/− fetal mice, whereas frequencies of Fancc^−/− weanlings were sub-Mendelian. χ² test comparing fetuses to weanlings yielded a P value of .004. (B) Representative photo of WT and Fancc^−/− day 14.5 fetal mice. Units are in millimeters. (C) Malformations observed in some Fancc^−/− fetuses: microphthalmia (left); anophthalmia and anencephaly (right). (D) Representative photos of 14.5 days post coitum WT and Fancc^−/− fetal livers, and (E) placentas. (F) Fetal mass normalized to WT mean within each litter (n_litters = 5, n_WT = 19, n_Fancc^−/− = 14). (G) Cell counts from single cell suspensions of fetal livers normalized to WT mean (n_litters = 6, n_WT = 16, n_Fancc^−/− = 13). (H) Placenta weights (n_WT = 25, n_Fancc^−/− = 14), P = .009. (I) Chromosome radial formation (%) in WT and Fancc^−/− fetal liver cells cultured in mitomycin-c (MM-C; n = 50/condition). Inset image of representative radial (left) and break (right). (J) FL and adult BM hematopoietic progenitor colony formation in methylcellulose (CFU-C) (n_FL = 3/genotype, n_BM = 1/genotype). Unfractionated cells were plated in the presence or absence of MM-C at 2 × 10⁴ per 35 mm dish and colonies of >50 cells were counted on day 12. (K) Mean percent difference in the secretion of select cytokines from Fancc^−/− vs WT fetal liver-conditioned media assayed by cytokine arrays in 3 independent experiments; the cytokines shown are those that yielded consistent results across all 3, whereas those that did not show differential expression are not shown. Error bars represent standard error of the mean. BM, bone marrow; FL, fetal liver; MM-C, mitomycin-c; WT, wild type.