Myr induces Tcl1 ubiquitination and degradation. (A) Daudi lymphoma cells were treated with CHX, Myr, and their combination for 24 hours. Cells lysates were analyzed by Western blotting with indicated antibodies. (B) Daudi lymphoma cells were treated with CHX, Myr, and their combination for 6, 24, and 48 hours in a time course experiment. The numbers above the blots indicate the intensity of the band expressed as ratio gene product TCL1/GAPDH and normalized to Mock. (C) HEK-293A cells were transfected with TCL1 and UB-HA plasmids. Forty-eight hours later, cells were treated with 17AAG, Myr, and their combination for additional 18 hours and MG132 for 3 hours; Total lysates were immunoprecipitated with anti-Tcl1. The immunoprecipitates were analyzed by immunoblotting with anti-HA and anti-Tcl1.