Production of proinflammatory cytokines is increased in lupus TC mice. (A) Serum levels of IL-6 were determined. Data were shown by symbols representing the value of each mouse. (B) Serum levels of IL-10 were shown by symbols representing the value of each mouse. (C) Expression levels of TNFα in BM cells were determined by qRT-PCR. cDNA input was normalized to the level of RNA polymerase IIa. Mean values ± SD obtained from at least 4 experiments are shown. (D) Expression levels of Mx1 in LSK cells measured by qRT-PCR are indicated as means ± SD (n = 3). *P < .05; **P < .01; ***P < .001. B6-O, old B6 mice at 10 months of age; B6-Y, young B6 mice at 8 to 10 weeks of age; TC-H, healthy TC mice at 8 to 10 weeks of age; TC-L, lupus TC mice at 10 months of age.