American Society of Hematology

Figure 5

$Figure 5. Sickle plasma activates ROS production in erythrocytes in a manner dependent on Rac-GTPase activity. (A) ROS production in fraction 1 SS RBC decreases significantly with incubation in ABO-matched AA plasma when compared with RBC incubated in endogenous or exogenous ABO-matched SS plasma. Cells were incubated for 24 hours in a standard tissue-culture incubator at 37°C before incubating with CM-H2-DCFDA for ROS quantitation. n = 20 and P < .05. (B) ROS production in fraction 1 AA RBC increases significantly with incubation in ABO-matched SS plasma compared with RBC incubated in endogenous or exogenous ABO-matched AA plasma. This induction is partially blocked by coincubation with NSC23766 (500 µM). Cells were incubated for 4 hours in a standard tissue-culture incubator before staining with CM-H2-DCFDA. n = 17 and P < .05.$

Sickle plasma activates ROS production in erythrocytes in a manner dependent on Rac-GTPase activity. (A) ROS production in fraction 1 SS RBC decreases significantly with incubation in ABO-matched AA plasma when compared with RBC incubated in endogenous or exogenous ABO-matched SS plasma. Cells were incubated for 24 hours in a standard tissue-culture incubator at 37°C before incubating with CM-H2-DCFDA for ROS quantitation. n = 20 and P < .05. (B) ROS production in fraction 1 AA RBC increases significantly with incubation in ABO-matched SS plasma compared with RBC incubated in endogenous or exogenous ABO-matched AA plasma. This induction is partially blocked by coincubation with NSC23766 (500 µM). Cells were incubated for 4 hours in a standard tissue-culture incubator before staining with CM-H2-DCFDA. n = 17 and P < .05.

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