Deletion of Raptor results in a reduction of migratory LCs. (A) FACS analysis of rDC (CD11c⁺ MHC-II⁺) and mDC (CD11c^int MHC-II^high) subsets in sLNs from Cre, rap^Δ/Δ, and ric^Δ/Δ mice. Populations shown in plots are gated on live cells. Bar graph displays mean frequency ± SEM of rDCs and mDCs (N = 17-21 mice per group). (B) Analysis of Langerin (CD207) and CD103 expression by mDCs in sLNs. Populations in plots are gated on live mDCs as shown in panel A. Numbers and bar graphs indicate mean frequency of cells in the respective quadrant ± SEM. Data were combined from 3 independent experiments with similar results (N = 6 mice per group). (C-D) DCs in the epidermis (C) and dermis (D) of enzymatically digested ear skin from Cre, rap^Δ/Δ, and ric^Δ/Δ mice. Populations in plots are gated on CD45⁺ cells (C) and CD45⁺ MHC-II⁺ CD11c⁺ cells (D). Numbers and bar graphs indicate the mean frequency of cells in the respective gate or quadrant ± SEM. Data were combined from 7 (C) and 4 (D) independent experiments with similar results (panel C: N = 15 mice for Cre, N = 13 mice for rap^Δ/Δ and ric^Δ/Δ; panel D: N = 8 mice for Cre and rap^Δ/Δ, N = 5 mice for ric^Δ/Δ).