Epidermal LCs of rap^Δ/Δ mice show a reduced expression of Langerin and β-catenin and a reduced cell size. (A) FACS analysis of epidermal LCs isolated by enzymatic digestion. Histograms show CD45⁺ MHC-II⁺ CD11c⁺ cells (LCs) from the epidermis of Cre (black line), rap^Δ/Δ (red line), and ric^Δ/Δ (green line) mice. Gray solid graphs show CD45⁺ non DCs (DETCs) in the histograms of CD207, CD324 and I-A/I-E or an isotype-matched control antibody staining in the histogram showing β-catenin expression. Bar graphs represent MFI ± SEM of N = 4 mice per group of the respective marker. Data were combined from 2 of 4 independent experiments with similar results. (B) FSC of epidermal and dermal DCs isolated by crawl-out (top panel) and mDCs and rDCs from sLNs (bottom panel) of Cre (black line), rap^Δ/Δ (red line) and ric^Δ/Δ (green line) mice. Cells were gated on CD45⁺ MHC-II⁺ CD11c⁺ (epidermis and dermis), MHC-II^high CD11c^int (mDCs) and MHC-II⁺ CD11c⁺ (rDCs). Data shown are representative of ≥ 3 experiments with at least 2 mice per group.