Raptor-deficient LCs have a higher frequency of proliferation and apoptosis. Analysis of proliferation (A), viability (B) and apoptosis (C-D) of epidermal DCs. DCs were isolated by enzymatic digestion (A) or by crawl-out from the epidermis (B-D) of Cre, rap^Δ/Δ and ric^Δ/Δ mice. Populations are gated on CD45⁺ MHC-II⁺ CD11c⁺/CD207⁺ cells. Numbers and bar graphs indicate the mean frequency ± SEM of cells within the respective gate or quadrant (A,C-D) or out of the gate (B). (A) Ki-67⁺ cells represent recently divided LCs. The very right plot shows staining with an isotype-matched control antibody. Data were combined from 2 independent experiments with similar results (N = 4 mice per group). (B) Violet-1⁺ cells represent dead DCs. One representative of 4 independent experiments with similar results is shown (N = 3 mice per group). (C) Annexin V⁺ 7AAD⁺ cells represent apoptotic DCs. Data are combined from 3 independent experiments with similar results (N = 6 mice per group). (D) VAD-FMK⁺ DAPI⁺ cells represent apoptotic DCs (N = 3 mice per group).