Progressive loss of Raptor but not Rictor-deficient epidermal LCs. (A) Microscopy of epidermal sheets from adult Cre, rap^Δ/Δ, and ric^Δ/Δ mice. LCs were visualized by MHC-II staining. The size bar corresponds to 50 μm. Original magnification ×40. (B) Epidermal sheets from adult Cre and rap^Δ/Δ stained with mAbs for MHC-II in red (LCs) and CD45.2 in green (DETCs and LCs). The size bar corresponds to 100 μm. Original magnification ×20. (C) Epidermal sheets from a day 8 (top panel) and a day 14 (bottom panel) rap^fl/fl litter were stained as in panel A. The size bar corresponds to 100 μm. Original magnification ×20. (D) Skin derived DCs isolated by crawl-out from whole ear skin of a day 5 (top panel) and a day 14 (bottom panel) rap^fl/fl litter. Populations shown were gated on CD45⁺ MHC-II⁺ CD11c⁺ cells. The mean frequency of CD207⁺ cells ± SEM of N = 2 to 4 mice per group is indicated under the gate and in the bar graph on the right.